Purwati, Nining (2021) Identification of endofit bacteria in kawista leaves (limonia acidissima l.). BIOEDUSCIENCE, 5 (1). pp. 85-91. ISSN 2614-1558

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Abstract

INDONESIA Background: Kawista (Limonia acidissima L.) merupakan tanaman buah tropis yang termasuk dalam suku jeruk-jerukan (Rutaceae). Limonia acidissima L. banyak dimanfaatkan secara tradisional oleh masyarakat di kabupaten Bima, Nusa Tenggara Barat. Namun Limonia acidissima L. belum banyak diketahui oleh masyarakat luas dikarenakan minimnya informasi yang mengkaji tentang tumbuhan ini. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri yang terdapat pada daun Limonia acidissima L. sebagai salah satu upaya untuk memperoleh informasi yang mendalam tentang tumbuhan Limonia acidissima L. Metode: Proses identifikasi dilakukan secara microbiology analyzer dengan bantuan alat identifikasi BD Phonix M50. Penelitian dilakukan di Balai Laboratorium Kesehatan Pengujian dan Kalibrasi Provinsi Nusa Tenggara Barat, dengan menggunakan daun Limonia acidissima L. yang diperoleh dari Kabupaten Sumbawa Barat. Hasil: Hasil penelitian menunjukkan sejumlah delapan isolat bakteri dari satu gram sampel daun Limonia acidissima L. berhasil ditemukan. Kesimpulan: Jenis bakteri yang ditemukan pada daun Limonia acidissima L. terdiri atas kelompok bakteri endofit, bakteri asam laktat, bakteri pereduksi metal, dan bakteri patogen. Kelompok bakteri endofit terdiri dari Bacillus sp. dan Bacillus megaterium. Kelompok bakteri asam laktat yaitu Leuconostoc mesenteroides spp. mesenteroides. Kelompok bakteri pereduksi metal yaitu Shewanella Putrefaciens sedangkan kelompok bakteri patogen terdiri dari Staphylococcus sp., Staphylococcus epidermidis, Staphylococcus capitis dan Streptococcus oralis. ENGLISH Background: Kawista (Limonia acidissima L.) is a tropical fruit plant that belongs to the Rutaceae tribe. Limonia acidissima L. is widely used traditionally by people in Bima district, West Nusa Tenggara. However, Limonia acidissima L. is not widely known by the public due to the lack of information that studies this plant. This study aims to isolate and identify the bacteria in the leaves of Limonia acidissima L. to obtain in-depth information about the Limonia acidissima L. plant. Methods: The identification process was carried out using a microbiology analyzer with the help of BD Phonix M50 identification tools. The research was conducted at the West Nusa Tenggara Province Health Testing and Calibration Laboratory, using the leaves of Limonia acidissima L. obtained from West Sumbawa Regency. Results: The results showed that a total of eight bacterial isolates from one gram of Limonia acidissima L. leaf were found. Conclusion: Eight types of bacteria found in the leaves of Limonia acidissima L. consist of a group of endophytic bacteria, lactic acid bacteria, metal-reducing bacteria, and pathogenic bacteria. The endophytic bacteria group consists of Bacillus sp. and Bacillus megaterium. The group of lactic acid bacteria, namely Leuconostoc mesenteroides spp. mesenteroides. The metal-reducing bacteria group is Shewanella Putrefaciens while the pathogenic bacteria group consists of Staphylococcus sp., Staphylococcus epidermidis, Staphylococcus capitis and Streptococcus oralis.

Item Type: Article
Uncontrolled Keywords: endofit bacteria; isolation; kawista leaves; limonia acidissima l.
Subjects: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060501 Bacteriology
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents
06 BIOLOGICAL SCIENCES > 0607 Plant Biology > 060705 Plant Physiology
Divisions: Fakultas Tarbiyah dan Keguruan > Jurusan Pendidikan Ilmu Pengetahuan Alam
Depositing User: Dr.,M.Pd Nining Purwati
Date Deposited: 18 Sep 2021 09:32
Last Modified: 13 Sep 2022 00:19
URI: http://repository.uinmataram.ac.id/id/eprint/597

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